Abstract

INHIBITOR OF ACETYLCHOLINE ESTERASE FROM HAMELIA PATENS FOR ALZHEIMER’S DISEASE BY USING DANIO RERIO AS A MODEL ORGANISM

Anisa Husna, Bhavya M., Manjushree R., Sushmitha G. C., Jayanth A., M. J. H. Shafi, and Guruprasad R.*

ABSTRACT

Acetylcholine esterase (AChE; EC 3.1.1.7) is an enzyme involved in the breakdown of the neurotransmitter acetylcholine into acetic acid and choline. Inhibition of AChE is needed to suppress neuro-degeneration in Alzheimer’s, Parkinson’s and other neurodegenerative diseases. Plants are known to contain AChE inhibitors which are used as antidote or anticholinergic poisoning. The methanolic extracts from leaves of Hamelia patens were screened using Gas Chromatography-Mass Spectrometry (GCMS) and was docked. The extracted leaves of the plant were checked for AChE inhibition on the brain of Danio rerio (zebrafish). The activity of the enzyme in in-vitro and in-vivo conditions is studied. The activity of the enzyme in in-vitro and in-vivo condition showed inhibition.

Keywords: Alzheimer’s disease, acetylcholine esterase, inhibition, Zebrafish, Plant extract, GCMS, Auto dock.