Abstract

IN VITRO ANTHELMINTIC ACTIVITY OF ACETONIC AND METHANOLIC EXTRACTS OF KHAYA SENEGALENSIS STEM BARK ON HAEMONCHUS CONTORTUS.

Tiropa Francis Chabi China, Sabbas Attindéhou*, Bienvenu Armand Gbangboché, Sahidou Salifou

ABSTRACT

In tropical areas, ruminants face serious problems with gastrointestinal parasites. Haemonchus contortus is a very worrying one. This study assessed the in vitro effects of acetonic and methanolic extracts of Khaya senegalensis stem bark against different development stages of Haemonchus contortus. Eggs, larvae, and adult worms of the parasite were exposed to gradual increasing concentrations (75, 150, 300, 600, 1200, and 2400 μg/ml) of the herbal medicine. Levamisole and Phosphate Buffered Saline (PBS) were respectively used as negative and positive control. From the results, the vegetal extracts induced over 75% of egg-hatching inhibition. Globally this ovicidal effect seemed not to be dose-dependent and the efficacy of both methanolic and acetonic extracts was well revealed at 150μg/ml concentration. On larval migration and adult motility the best effectiveness were registered with the methanolic extract. The lethal effect was dose-dependent and 75% or 100% of worms died within 24h when exposed respectively to 2400μg/ml of the acetonic extract or to 1200μg/ml of the methanolic extract. K. senegalensis extract could be a judicious alternative to chemical drug against Haemonchosis in sheep and goats from endemic areas like Africa.

Keywords: Haemonchus contortus – Khaya senegalensis, extracts - Anthelmintic efficacy.