Abstract

CHROMATOGRAPHIC FINGERPRINT ANALYSIS OF VITEX ALTISSIMA LINN LEAF EXTRACT BY HPTLC TECHNIQUE.

Thenmozhi S.*, Varadharaju S., Vijeesha Raju, Rafiya, Syed Jalal Urosa Eram and Syed Jalal Ummae Aeman

ABSTRACT

Aim and objective of the study, to determine the flavonoids, saponins, steroids and phenolic compounds of hydroalcoholic extract of Vitex altissima Linn. (Synonym: Milla) leaves using HPTLC technique. The extract was tested to find out the presence of various phytoconstituents like carbohydrates, alkaloids, flavonoids, saponins, terpenoids, steroids, tannins, protein and aminoacids. A CAMAG HPTLC system equipped with LINOMAT 5 applicator, a TLC scanner 3, REPROSTAR 3 and WIN CATS-1.3.4 software were used. The mobile phases in the different compositions were used for high resolution. The results of qualitative phytochemical screening confirm the presence of carbohydrates, phenols, saponins, flavanoids, terpenoids, steroids and tannins. The extract showed the presence of 13 different types of flavonoids with 13 different Rf values in the range of 0.01 to 0.97. The results of saponin illustrated the presence of 12 types of saponins with 12 types of Rf value ranging from 0.01 to 0.92. The results of phenolic profile showed the presence of 15 types of phenolic compounds with 15 different Rf values in the range of 0.02 to 0.94 and the results of steroid profile demonstrated the presence of 7 types of steroids with 7 types of Rf value range from 0.02 to 0.53. It can be concluded that the present study used to evaluate the medicinal plant Vitex altissima from the adulterant. HPTLC fingerprint analysis developed to help in proper identification and quantification of marker compounds. By isolating and identifying the marker compounds, new drugs can be formulated to treat various diseases.

Keywords: Vitex altissima, HPTLC, Flavonoids, Saponins, Phenols, Steroids.