Abstract

CALLUS INDUCTION AND REGENERATION FROM NODAL EXPLANTS IN TRIBULUS TERRESTRIS LINN., AN IMPORTANT MEDICINAL PLANT

*Manogya Singh and S.C. Goyal

ABSTRACT

Tribulus terrestris Linn. is an annual creeping herb. In India, it is used as a medicine against impotency and cardiovascular disease. In vitro studies were conducted with employing different explants viz. leaf, internode and node used for callogenesis and differentiation. Among different explants viz. leaf, internode and node used for callus induction and growth, node explant was found to be the best for induction and growth of callus. Early response with best callus induction (100%) was obtained on Murashige and Skoog’s medium fortified with 1.0 mg l-1 2,4-dichlorophenoxy acetic acid (2,4-D) in combination with 0.5 mg l-1 kinetin (Kn) from node explant. Therefore, this combination was found superior for callogenesis. Direct regeneration of shoots from nodal explants was observed on MS medium supplemented with combinations of 3.0 mg l-1 benzyl amino purine (BAP) and 2.5 mg l-1 naphthalene acetic acid (NAA). Regenerated shoots were rooted on half strength MS medium supplemented with concentrations of 0.1, 0.2 and 0.5 mg lindole butyric acid (IBA). Among the different combination used, maximum number of roots appeared on half strength MS medium supplemented with 0.1IBA (mg/l) within 10 days after inoculation with 98.3% success.

Keywords: Tribulus terrestris, callus, regeneration.