Abstract

EVALUATION OF HYBRID PROTEINS WITH APOPTOSIS ACTIVITY (A1-GM CSF) RECEPTORS ON VARIOUS CELL LINES IN IN VITRO GMCSF

Elnaz Heydari*

ABSTRACT

One of the new solutions targeted to treat diseases such as cancer, the use of substances is known as immunotoxins. Immunotoxins consist of two parts tracking and exterminators. In recent years a new mechanism has been established for this purpose and the use of a cytotoxic molecule that is carried out by the fusion technology. In this study, a hybrid protein that is composed of two catalytic Shiga toxin A1 and GMCSF is human, and cytotoxic activity induced by arabinose GMCSF receptor on cells was tested. The data indicate that the hybrid proteins were cytotoxic to GMCSF receptor cell lines and different concentrations of the proteins induces apoptosis.

Keywords: apoptosis, protein GM CSF, immunotoxins, cancer.