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DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF METHOCARBAMOL AND DICLOFENAC SODIUM IN INJECTION DOSAGE FORM
Nikita P. Patel* and Madhuri Hinge
ABSTRACT A simple, precise and accurate liquid chromatographic method was developed for simultaneous determination of methocarbamol and diclofenac sodium in injection dosage form. The chromatography was carried on BDS Hypersil C18 (250 mm x 4.6 mm, 5μm) column and with Mobile phase Composition of Phosphate buffer: Methanol (30:70 v/v), pH 4.5 with Orthophosphoric acid at a Flow rate of 1ml/min and detection was carried out at 281 nm. RP-HPLC method was found to be linear over the range of 3-9 μg/ml for Diclofenac sodium and 18-54 μg/ml for Methocarbamol. Retention time of Diclofenac sodium and Methocarbamol was found to be 4.2501 and 6.7832 respectively. Correlation Co- efficient of Diclofenac sodium and Methocarbamol was found to be 0.9968 and 0.9945 respectively. The method has been validated for Linearity, Precision, Accuracy, LOD, LOQ and System suitability according to ICH Q2 (R1) guideline. Keywords: Methocarbamol, Diclofenac sodium, RP-HPLC. [Download Article] [Download Certifiate] |