Abstract

FIVE SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF ETILEFRINE HCl IN BINARY MIXTURE WITH CHLORPHENIRAMINE

Khalid Abdel-Salam M. Attia, Nasr M. El-Abasawy and Ahmed M. Abdelraoof*

ABSTRACT

Five Simple, rapid, sensitive, accurate and precise stability-indicating spectrophotometric methods were developed for the determination of Etilefrine hydrochloride (ETF) in bulk powder and in pharmaceutical preparation. Method (A) Ratio derivative method (1DD), Method (B) Ratio difference method, Method (C) Mean centering method, Method (D) Dual wavelength method and Method (E) Area under the curve (AUC). These methods were used for the determination of Etilefrine hydrochloride in binary mixture with (chlorophenermine maleate) (CPM). These methods were validated and successfully applied to the determination of Balkis ® capsule with an average percent recovery ± RSD% of 100.26± 0.622 for method (A), 99.73 ± 0.583 for method (B), 99.15 ± 0.670 for method (C), 99.63 ± 0.862 for method (D) and 99.41 ± 1.225 for method (E). The obtained results were statistically compared with those of the reported method by applying t-test and Ftest at 95% confidence level and no significant difference was observed regarding accuracy and precision.

Keywords: Etilefrine, Ratio derivative, Ratio difference, Mean centering, Dual wavelength, Area under curve.