Abstract

RP-HPLC METHOD FOR ESTIMATION OF ETORICOXIB AND THIOCOLCHICOSIDE FROM TABLET DOSAGE FORM

Dr. Pillai Sujit* and Deshmukh Nitin

ABSTRACT

A rapid, simple and sensitive RP-HPLC method was developed for the simultaneous estimation of Etoricoxib and Thiocolchicoside in tablet dosage form using C-18 column (250 mm, 4.6 mm, 5μ) RP column and Acetonotrile: 0.05M ammonium acetate (80:20) pH 6.5 as mobile phase at flow rate of 1.0 ml/min. A 240 nm UV detection wavelength was used. The calibration curve was plotted and was linear at 2-40 μg/ml for both Etoricoxib and Thiocolchicoside with retention time 5.01 min and 9.04 min respectively. Caffine was used as internal standard and 240 nm was selected as detection wavelength. The developed method was validated satisfactory and by recovery studies.

Keywords: Etoricoxib, Thiocolchicoside, RP-HPLC, validation.