Abstract

PREVALENCE, ANTIMICROBIAL RESISTANCE PATTERN AND VIRULENCE PROPERTIES OF E.FAECALIS ISOLATED FROM URINE SAMPLES IN NAMAKKAL AREA.

M. Suganthi, L. Ashok Kumar* and D. Jegadeesh Kumar

ABSTRACT

In this study, 30% of E.faecalis was observed from 50 urine samples of different age groups. The highest occurrence was observed in female than male patients. E.faecalis was subjected to antibiotic susceptibility test with seven antibiotics, among them vancomycin and erythromycin showed highest antibiotic resistance and ciprofloxacin was active against most of the isolates. In this study, 12 types of antibiotic resistance patterns were observed. This study has been conducted to investigate the occurrence of virulence factors like biofilm (80%), betalactamase (73.3%) and gelatinase (53.3%). In this study, two virulence genes were investigated in eleven clinical isolates of E. faecalis by using PCR technique. Among them, 64% were harbored the cytolysis gene and 9% were harbored the esp gene. This study showed an emergence of VRE along with increased rates of multidrug-resistant enterococci in the area of the study. Furthermore, this study also attempted to determine if there is any significant difference in the biofilm positive isolates and biofilm negative isolates.

Keywords: E.faecalis, Biofilm, Betalactamase, gelatinase, cytolysin.