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Abstract

FINGER PRINT ANALYSIS OF ETHANOLIC EXTRACT OF AMORPHOPHALLUS CAMPANULATUS DECNE TUBER.

Seema Firdouse*, Dr. Jyoti Gupta and Parwez Alam

ABSTRACT

A selective HPTLC analytical method has been developed for the finger printing of Amorphophallus campanulatus tuber. Initially dried powder of Amorphaphallus campanulatus tuber was extracted with ethanol, and a new solvent system has been developed for the best separation of phytoconstituents present in the extract. The solvent system selected for HPTLC was Ethanol: Ethyl Acetate: Glacial Acetic Acid (7 : 3: 0.1)v/v, stationary phase was precoated silica gel aluminium plate 60 F254 (5 cm ×10 cm) with 250 μm thickness. The separated bands on the HPTLC plates were scanned over the wavelength of 200 - 400 nm.

Keywords: Amorphophallus campanulatus, Ethanolic extract, HPTLC.


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