Abstract

CELL GROWTH INHIBITION AND APOPTOSIS BY EXTRACT OF BASELLA ALBA PLANT ON U937 CELLS

Krishnendu Pal*

ABSTRACT

Leukaemia is one of the leading causes of death in the world particularly developing countries & it is complex group of disease with many possible cause. The synthetic drug and chemotherapies have huge side effects and these are so costly treatment for middle and lower class families. It would be extremely beneficial if an anticancer drug can be discovered from natural edible sources. Therefore our plant of interest an edible plant Basella alba reported as a natural medicinal source on the basis of its potent antibacterial and antioxidant, antiulcer, antidiabetic, hepatoprotective activity. Present study is the evaluation of anti-proliferative effect of the extract & active fraction of Basella alba plants in U937 cell line. Cell viability was studied by Trypan blue exclusion and cell Cytotoxicity study by MTT assay, Morphological study by phase contrast microscopy. DNA laddering assay by gel electrophoresis shows its apoptotic activity. MEBA were found to be responsible for antiproliferative, cytotoxic activity and the morphological images, DNA laddering assay were found to be responsible for the apoptosis of cells. The extract of Basella alba plant was found to be having potent anti-leukemic activity on U937 cells. It can be expected in future to develop an anti-cancer compound for the treatment of human from natural source with less systemic toxicity. Thus, further more investigation is needed for mechanism and to identify the active compound responsible for this activity.

Keywords: Basella alba, Leukaemia, Myeloid, apoptosis