Abstract

STUDY OF ANTIOXIDANT ACTIVITY OF CEE'RICH - HERBAL VITAMIN C SUPPLEMENT IN EXPERIMENTAL ANIMALS.

Vijayalaxmi Hiremath, Supriya CS, Khader Shareef KS, Nataraj Loganayaki, Shanaz Tejani-Butt, Shivalinge Gowda KP*, K. Venkateswarlu.

ABSTRACT

The antioxidant activity of Cee’Rich Vitamin C supplement was investigated using carbon tetrachloride (CCl4, 2ml/kg s.c route) intoxicated albino Wistar rat liver. The Cee’Rich Vitamin C supplement was administered orally at a dose of 200 and 400mg/kg, for 10 consecutive days. The doses were determined from the acute oral toxicity study in albino mice. The CCl4 was administered to all the groups except group I (normal control). Silymarin (100 mg/kg) was used as a standard hepatoprotective drug. After 24h of the last treatment, blood was withdrawn by retro orbital puncture and serum SGPT, SGOT and LDH were determined. The SOD was calculated from the liver homogenate. The results indicated that serum SGPT, SGOT and LDH levels were significantly decreased where as SOD levels were significantly increased in Cee’Rich Vitamin C treated rats. This study suggests that the presence of flavonoids in the Cee’Rich Vitamin C supplement exhibit significant hepato protective effect and could protect tissues from oxidative stress via a free radical- scavenging and antioxidant mechanism.

Keywords: Cee’Rich Vitamin C supplement, Carbon tetrachloride, Superoxide dismutase, Lactate dehydrogenase, Antioxidant enzymes, Silymarin and Flavonoids.