Abstract

EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF COPPER(II) BY USING 2-HYDROXY-3-METHOXY BENZALDEHYDE THIOSEMICARBAZONE AS AN ANALYTICAL REAGENT

*Y. K. Patil, R. S. Lokhande, P. K. Rana, S. P. Janwadkar, C.Y. Patil

ABSTRACT

2-Hydroxy-3-methoxy Benzaldehyde Thiosemicarbazone (2H3MB TS) as a photometric reagent for the extractive spectrophotometric determination of Copper (II) is presented in this paper. The reagent 2H3MBTS gave instantaneous and stable blue colour with Copper (II) in the pH range 6.2 to 7.6. The system obeys Beer’s law in the range in the concentration range of 1.0 μg/mL to 10.0 μg/mL at 420 nm. The Limit of Detection (LOD) is calculated by using standard deviation of blank solution at confidence limit 98.3%, which is found to be 0.327 μg/mL. The stoichiometry of the complex is established as 1:2 (M: L) by Job’s method of continuous variation and confirmed by mole ratio method. The standard deviation and the coefficient of variance are presented. The results of the prescribed procedure applied for the determination of the micro amounts of Cu (II) in standard steel samples, alloys, pharmaceutical and synthetic samples are presented.

Keywords: Copper, Spectrophotometric determination, n-Butanol, 2-Hydroxy-3-Methoxy Benzaldehyde Thiosemicarbazone.