Abstract

ANTINEOPLASTIC ACTIVITY OF QUERCETIN, IN-VITRO STUDIES ON HL-60 CELL LINE AND IN-VIVO STUDIES ON DAL INDUCED MICE

Swati Som*, Manjunatha P. M., T. Prakash and Divakar Goli

ABSTRACT

Objective: Evaluate antineoplastic activity of Quercetin in-vitro on HL-60 cell line and in-vivo against Dalton’s ascitic lymphoma (DAL) induced Swiss albino mice. Methods: Cytotoxic effect of Quercetin was measured by MTT assay on HL-60 leukemic cells and therapeutic efficacy of Quercetin in-vivo was evaluated against DAL induced Swiss albino mice. Group-I served as normal control and remaining groups were induced with Dalton’s lymphoma by injecting 1×106 cells/ml/mouse intraperitoneally. Quercetin 15 mg/kg and 30 mg/kg were administered orally for 15 days, after treatment period blood was collected from animals for biochemical and hematological investigations. Subsequently they were sacrificed for isolation of liver further subjected for evaluating antioxidant status. Other set of 5 groups of 6 mice each were continued with the treatment daily for observing life expectancy. 5-Flurouracil (20 mg/kg) was used as a standard drug. Results: Quercetin shown cytotoxicity for HL-60 cells in dose dependent manner and treatment with 15 mg/kg and 30 mg/kg doses of Quercetin in DAL induced mice significantly (P

Keywords: Quercetin, Dalton’s ascitic lymphoma, HL-60, 5-Flurouracil.