Abstract

CHARACTERIZATION OF BIOSYNTHESIZED SILVER NANOPARTICLES AND THEIR EFFECT ON MULTI DRUG RESISTANT BACTERIA AND ACUTE LYMPHOBLASTIC LEUKEMIA CELLS

Jaafar Faez Kadhim, Rasheed M. U.*, Zaid I.I Al-Luhaibi, Raid-Al-Akeel, Rabbani Syed and Kaiser Jamil

ABSTRACT

Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. There is a growing demand to develop eco-friendly techniques for Silver Nanoparticles (Ag-NPs). The aim of this study was to develop low cost methodology for silver nanoparticles to meet their demand for their wide applications. Further to characterize them and evaluate their properties as bioactive agents. The leaf extract of Azadirachta indica was used for reduction of silver ions using silver nitrate as reducing agent. The biosynthesized Ag-NPs were characterized by UV-VIS Spectrophotometer, FTIR, XRD, SEM and TEM. These were tested in-vitro for their antibacterial activity against multi drug resistant bacteria and cytotoxic effect on acute lymphoblastic leukemia (ALL) cells. XRD, SEM and TEM analysis confirmed that that most of the Ag nanoparticles were spherical in shape with crystalline structure and had the size in the range of 2 to 50 nm. The FTIR spectra revealed the presence of functional groups which are responsible for the capping and stabilization of the synthesized silver nanoparticles. Ag-NPs were active against leukemia cells in MTT assay as well as effective against super bugs like ESBL and MBL producers as well as MRSA. Ag-NPs may prove as an alternative in manufacturing pharmaceutical products and medical devices that may help to prevent the transmission of multi drug-resistant pathogens and combat some types of cancers. But toxicological limitations for eukaryotic cells should be taken in account since nanosilver is not a target specific antibacterial/antileukemic agent.

Keywords: Silver Nanoparticles, TEM, Multi Drug Resistant Bacteria, Acute Lymphoblast Leukemia, ESBL, MRSA.