Abstract

ANTIOXIDANT AND ANTICANCER POTENTIAL OF MANGOSTEEN FRUIT, GARCINIA MANGOSTANA AGAINST HEPATOCELLULAR CARCINOMA (HePG-2) CELL LINE

Manimekalai I., Sivakumari K.*, Ashok K. and Rajesh S.

ABSTRACT

The antioxidant and anticancer potential of mangosteen fruit Garcinia mangostana have been unraveled in the present study. Extraction of the epicarp and endocarp of the fruit with methanol, chloroform, hexane and ethyl acetate revealed that methanol gave maximum yield of extract. However, standardization of the solvents by MTT assay showed that only chloroform extract of epicarp and hexane extract of endocarp had significant effect on HeP-G2 cells, thus revealing that the hexane extract has profound effect on HeP-G2 cells. In toto, it can be concluded that both the epicarp and endocarp of Garcinia mangostana have antioxidant and anticancer potential. Moreover, identification of active phytoconstiuents in the extracts will pave a way for using this fruit as a natural cytotoxic agent against various cancers.

Keywords: DPPH assay, ORAC assay, MTT and cell cycle analysis.