ISOLATION AND CHARACTERIZATION OF RHIZOSPHERIC BACTERIA IN SOYBEAN (Glycine max (L.) Merrill) CULTIVATED ON FERRALSOLS OF BUONHO TOWN, DAKLAK PROVINCE, VIETNAM
*Prof. Dr. Cao Ngoc Diep, Nguyen Ba Trung and Dr. Van Thi Phuong Nhu
ABSTRACT
Rhizobacterial diversity and population dynamics in the ferralsols rhizosphere of soybean grown in BuonHo town, DakLak province, highland of Vietnam was studied. Soil rhizosphere samples were taken in 24 sites of this region. Physical and chemical characteristics of soil samples and total nitrogen-fixing and phosphate-solubilizing bacteria counts were determined by drop plate count method together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (8F and 1492R). A total of 43 isolates were isolated on two media (Burk’s N-free and NBRIP) and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis. Population of rhizobacteria correlated with soil pH and organic matter content in soil closely (P
Keywords: 16S rRNA, Ferrasols, Gene Sequence, Rhizobacteria, Rhizosphere, Soybean.
[Download Article]
[Download Certifiate]