Abstract

A STUDY ON QUANTIFICATION OF PHYTOCONSTITUENTS AND IN VITRO ANTIFUNGAL ACTIVITY OF S.CUMINI AND S.JAMBOS LEAF EXTRACTS

Devakumar J., Sudha S. S.*, Keerthana1, Anitha S. and Ezhilarasi R.

ABSTRACT

Background: Dissemination of fungal spores emerges as a major problem in the spread of spore derived allergic pulmonary diseases. Fungal infections with multidrug resistances have posed a great threat in tropics and emerge with resistance to antifungal agents. Phytotherapy is an arena practiced among the folklore traditional healers for various diseases and disorders. Objectives: This study was aimed to quantify the phytoconstituents and to determine the antifungal activity of acetone and aqueous extracts of Syzygium sps,. Material and methods: Among the two solvents utilized for the study acetone was found to be the best phytoconstituent extractor as compared to aqueous and higher percentage of antifungal activity was observed with acetone extracts of S.jambos as compared to S.cumini. Results: The most significant 100% inhibition was observed on SCACE against C.neoformans and T.rubrum and SJACE against C.neoformans with MIC at 500μg/mL and 250μg/mL with their IC50 values from 291 μg/mL, 52.67 μg/mL and 34.36 μg/mL respectively. In addition, 80-100 μg/mL at MIC >1000 μg/mL was observed to be the inhibitory level of SJACE against all five fungus pathogens and considerable IC50 values were noted. Conclusion: Acetone was more effective for the extraction of Phytoconstituents and antifungal components from leaves of Syzygium sps.

Keywords: Antifungal activity, Phytoconstituents, Acetone extract, aqueous extract, phenolic content, Alkaloid, Flavonoid, Saponin.