Abstract

IN-VITRO ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE INHIBITION ACTIVITY OF UMBELLIFERONE AND BETA-IONONE ISOLATED FROM CORIANDRUM SATIVUM LINN.

Ayyasamy Rayar* and Rajamanickam Manivannan

ABSTRACT

Aims: The objective of the present study was to provide an In‑Vitro evidence for the potential inhibitory activity of Ethanolic extract, Umbelliferone and β-Ionone on α‑amylase and α‑glucosidase enzymes. Materials and Methods: Differentconcentrations (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) of Ethanolic extract, Umbelliferone and Beta-Ionone were subjected to α-amylase and α-glucosidase inhibitory assay. The absorbance was measuredat 540 and 405 nm using multiplate reader and the percentage of α‑amylase and α‑glucosidase inhibitory activity and IC50 values of Ethanolic extract, Umbelliferone and Beta-Ionone were calculated. Result and Discussion: Ethanolic extract of Coriandrum sativum Linn. has shown highest α –amylase and α-glucosidase inhibitory potential with IC50 values of 0.294, 0.211 which was comparable with acarbose (0.125 and 0.93 mg/ml). Whereas, Umbelliferone and 𝛽-Ionone have shown lesser activity. Conclusion: The results of the present study indicate that, Ethanolic extract of Coriandrum sativum Linn, rich in triterpenoids and phenolics is effective α‑amylase and α‑glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels. Hence, it may useful in the management of T2D.

Keywords: Coriandrum sativum Linn, Umbelliferone, ????-Ionone, ?-amylase and ?-glucosidase.