Abstract

APOPTOSIS EFFECT OF ISOLATED PHENOLIC SECOIRIDOID GLUCOSIDE FROM AERIAL PARTS OF GOMPHRENA GLOBOSA IN A-431CELL LINES

Latha ST*¹, Rajendran NN²

¹Department of Pharmaceutical Chemistry, Swamy Vivekanandha College of Pharmacy,Tiruchengode, 637205

²Department of Pharmaceutics, Swamy Vivekanandha College of Pharmacy, Tiruchengode,637205

ABSTRACT

The phenolic secoiridoid glucoside (Oleuropein) isolated from chloroform extract of aerial parts of Gomphrena globosa was evaluated for the apoptosis effect responsible for cytotoxic activity on A-431 cell lines. The isolated compound was elucidated by spectral characterization and was already reported. The presence of Osubstituted dihydroxy phenolic group may be responsible for the activity. The possible mechanism of apoptosis was studied by LDH Leakage assay, DNA fragmentation and caspase -3 activity and was found out that the apoptosis may be through killing the target cell by cytotoxic T- lymphocyte and natural killer cells and caspase-3 dependent apoptosis.

Keywords: Gomphrena globosa, cytotoxic activity, apoptosis, LDH leakage assay, caspase -3 activity, phenolic secoiridoid glucoside, A-431 cell lines.