Abstract

BIOASSAY OF HISTAMINE BY USING ISOLATED CHICKEN ILEUM

Rakesh B. Daude*, Soniya V. Mishra, Raosaheb Y. Ghegade and Sunil V. Amrutkar

ABSTRACT

Study of isolated organ/tissue preparation study has to sacrifice laboratory animals just for teaching purpose. The objective of present investigation was to study effect of histamine on dose response curve (DRC) and to find out unknown concentration of histamine by interpolation and three-point bioassay using isolated chicken ileum. The results suggested that histamine produced a dose dependent increase in response of contraction using tyrode physiological salt solution (PSS). Unknown concentration matches nearby with actual concentration by interpolation and three-point bioassay. The result shows that the chicken ileum is suitable for performing bioassay of histamine, an alternative to isolated ileum preparation from laboratory animal (guinea pig) without sacrificing the experimental animals.

Keywords: chicken ileum, histamine, interpolation, three-point bioassay.