Abstract

CHEMICAL COMPOSITION AND IN VIVO ANTI-INFLAMMATORY ACTIVITY OF THE LIPID EXTRACT FROM PREMNA ODORATA BLANCO CULTIVATED IN EGYPT

Abeer H. El-Mudomy, Hossam M. Hassan*, Elham Amina, Waleed A. Mohamed and Mona H. Hetta

ABSTRACT

Premna odorata Blanco (Labiatae) popularly known as ―alagaw‖ is a tree native to temperate and tropical Asia including the Philippines. Reviewing the literature; nothing could be traced discussing lipid content or evaluating the biological activity of this plant. This study was aimed to identify the chemical composition of P. odorata lipoidal extract using GC/MS analysis. and evaluating its anti-inflammatory activity by measuring the pro-inflammatory markers (COX-II and IL-6) in Inflamed liver Wister albino rats. Twenty five compounds were identified by GC/MS analysis representing 72.15% of total identified compounds consisted mainly of fatty acids in free and ester forms (57.18%). Linolenic acid and its ester form were the main identified compounds representing 18.58% and 19.00% of the total identified compounds, respectively. Biological results showed that treatment of Inflamed liver of rats using a dose of 500mg/kg lipid extract had insignificant level change in COX-II and IL-6 when compare to normal control group. It could be concluded that P. odorata lipid content consisted mainly of fatty acids and interferes with COX-II and IL-6 roles during the inflammation progress disease.

Keywords: Premna odorata; lipoidal; anti-inflammatory; GC/MS; Linolenic acid.