Abstract

IN VITRO ANTIMICROBIAL STUDIES OF EUCALYPTUS METHANOL EXTRACT AGAINST MAJOR SKIN PATHOGENS

*Udgire M.S. ¹, Pathade G.R. ²

ABSTRACT

The antibacterial activity of the methanol extract of Eucalyptus camaldulensis against major skin pathogens were investigated using agar-gel diffusion techniques. The MIC was obtained with the crude extract against each of the pathogens. The results obtained showed that methanol extract exhibited broad spectrum antimicrobial activities against all test pathogens. The leaf extract produced inhibition zone ranging from 10.0 – 20.1 mm against the test pathogens. The methanol extracts of the test plant produces larger zones of inhibition against the gram negative bacteria. The minimum inhibitory concentration (MIC) range observed was 200-400 μl/mL. The methanol leaf extract was analyzed by Gas Chromatography Mass Spectrometry (GC-MS). The Major components identified in the extract were documented as eucalyptol (44.22%), and α- Pinene (13.60%) followed by β pinene (2%), 3-Carene(5.05%), 0-Cymene(6.20%), gamma- Terpinene (3.45%), and alpha- Terpineol (6.01%) with some other trace components which contributed for their antimicrobial activities.

Keywords: Eucalyptus camaldulensis, Methanol extract, Antimicrobial Activity, GC-MS.