Abstract

MICONAZOLE LOADED NOVEL PHYTOSOMAL TOPICAL GELS

Prasuna Sundari Pingali*, Prathima Srinivas and B. Madhava Reddy

ABSTRACT

Phytosomes are phospholipid vesicles advocated for enhanced delivery of polar biomolecules. Phosphatidylcholine-complexes of Mitomycin, Clarithromycin, silybin, rutin, 10-hydroxycamptothecin, oxymatrine, luteolin have been successfully formulated and evaluated demonstrating the merit of the novel formulation.The aim of the present work was to formulate and evaluate miconazole loaded topical phytosomal gels. This study attempts the use of phytosome as carrier for drug loading of Miconazole(MCZ). Schrebera swietenioides root bark has high polyphenol content and can form phytosomal complexes with soyalecithin. Schrebera root bark extract (SRE) was used to formulate miconazole loaded phytosomal complexes (MPC’s). Schrebera swietenioides (Oleaceae) root bark methanolic extract (SRE) was prepared by soxhlation. Miconazole nitrate (MCZ) and SRE were complexed using soyalecithin in definite drug: lipid ratios by ethanol method. All Miconazole loaded phytosomal complexes were evaluated and characterized for entrapment efficiency, in vitro release, vesicle size, vesicle stability and SEM. Blank phytosomes (SRE-soyalecithin complex) and drug-loaded phytosomes (MCZ loaded SRE phytosomes) were included for comparison. Further, the optimised MPC’s were formulated as gels using varying polymers in differing concentrations and were assessed for their homogeneity, pH, drug content and in vitro permeation using Franz diffusion cell. The optimized formulation F4 was subjected to stability studies as per ICH guidelines. Microscopical examination of MPC’s revealed spherical shape and uniform size. MP1B and MP6B were optimized based on their in vitro drug release profile. The evaluation of prepared phytosomal gels of MP1B and MP6B indicated F4 to be better with 93.3 % drug content and 92.54% cumulative drug release of MCZ in 12hrs. Miconazole was successfully loaded into the SRE-phytosomal complex. The results demonstrated that other poorly soluble drugs can be further be explored for better therapeutic benefit.

Keywords: Miconazole, Schrebera swietenioides, phytosomal complex, topical phytosomal gel.