Abstract

IN-VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF CLERODENDRUM COLEBROOKIANUM WALP LEAVES

M. Ayub Ali, C. Lalmuanthanga, C. Lalchhandama and L. Inaotombi Devi*

ABSTRACT

Clerodendrum colebrookianum Walp. is a perennial shrub belonging to the family Verbenaceae. Globally the species is distributed in Bangladesh, Bhutan, China, Indonesia, Malaysia, Nepal, Sri Lanka and Vietnam. In India it is distributed in the states of Assam, Meghalaya, Sikkim, Mizoram and Manipur. Clerodendrum colebrookianum Walp. has been considered as the most important medicinal species which is used in the treatment of hypertension by different tribes of North East India. Mizos use this as vegetable and it is belief that there is low percentage of hypertensive people in their community as a result of regular intake of this as vegetable. The fresh leaves of the plant were washed, air dried in shade, ground to powder and subjected to cold maceration using methanol as per the standard procedure, condensed by subjecting to a rotary evaporator and lyophilized. The methanolic extract prepared was analyzed for antioxidant activity by three different in vitro methods; namely (1) DPPH free radical scavenging activity, (2) Ferric reducing antioxidant potential (FRAP) assay and (3) Total phenolic contents by spectrophotometeric method. In the radical scavenging measurement method of DPPH and ferric iron reducing ability assay, the antioxidant activities were 31.87±0.07 mg and 14.12±0.165 mg Trolox equivalent respectively per gram of dried leaves while, the total phenolic content, which is said to have a direct relevance with antioxidant activity was determined to be 2.47±0.08 mg GAE per gm of dried leaves.

Keywords: Clerodendrum colebrookianum Walp. leaves, antioxidant activity, DPPH, FRAP, Total phenolic.