Abstract

SIMULTANEOUS ESTIMATION OF AZITHROMYCIN AND CEFPODOXIME PROXETIL FROM ITS TABLET DOSAGE FORM BY UV VISIBLE SPECTROSCOPIC METHODS.

Mirza Shahed Baig*, Mohammed Imran Anees, Khateeb Muntasiruddin, Shaikh Naeem, Javed Khan

ABSTRACT

New simple, precise, accurate and cost effective UV spectrophotometric methods are developed for the estimation of azithromycin and cefpodoxime proxetil from its combined tablet dosage form by simultaneous equation method and derivative spectroscopic method. Both the method utilized methanol as solvent. Method – I is simultaneous equation method in which wavelength selected for azithromycin is 251 nm (λmax) and cefpodoxime shows maximum absorption at 234 nm. Method – II is derivative spectroscopic method in which zero order (D0) and first order (D1) derivative spectroscopic methods were explicated. Zero order derivatives utilize λmax of both the drugs as 251 nm and 234nm for analysis. While first order derivative spectroscopic method uses 233 nm (zero crossing point of cefpodoxime) for azithromycin and 245 nm (zero crossing point of azithromycin) for cefpodoxime analysis. Both the drugs follow beer-lamberts law in the concentration range of 5-30 μg/ml. The correlation coefficient of azithromycin and cefpodoxime was found to be as 0.999. Percent recovery for both the drugs is found to be around 99.95 %. Both the developed UV spectrophotometric methods were validated according to ICH guidelines with respect to accuracy, precision, ruggedness, specificity, LOD and LOQ. Both the proposed methods can be implemented for routine analysis of azithromycin and cefpodoxime in its combined tablet dosage forms.

Keywords: Azithromycin, Cefpodoxime, UV Spectroscopy, Simultaneous equation method, First order derivative.