Abstract

SCREENING OF EFFECTIVE SUBSTRATE FOR THE PRODUCTION OF CELLULASE BY TRICHODERMA HARZIANUM

Manglanayaki R.* and Madhavan S.

ABSTRACT

The production of cellulolytic enzymes by Trichoderma harzianum in submerged culture with banana leaf, banana stem and banana rhizome as substrates was studied. Effects of some factors, such as substrate concentration, pH, temperature and incubation time were reported. Optimal cellulase secretion by Trichoderma harzianum was using banana leaf produce high amount of enzyme when compare to stem and rhizome The production of cellulases by Trichoderma harzianum in submerged fermentation was investigated CMC served as a powerful inducer that enhance the cellulases yield where glucose repressed cellulase activates of incubation time 9th day (0.45 IU/ml), pH 4 (0.61 IU/ml), Temperature 45°C (0.40 IU/ml) and substrate concentration 3 % (0.76 IU/ml) respectively. Using low – cost agro industrial waste for production of cellulose in submerged fermentation would help to reduce the cost of enzyme production and expand the application field of cellulose. The crude enzyme when purified may serve the importance of this enzyme in both refining and deinking of recycled papers.

Keywords: Trichoderma harzianum, banana leaf, banana stem, banana rhizome and cellulose.