Abstract

COMPARATIVE EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF OLEOGUMRESIN & STEMBARK EXTRACT OF GUGGULU IN ALBINO RATS.

Dr. Malviya Reeta*, Dr. Sharma Ravindra, Dr. Sharma Tarun, Dr. Ramamurthy A., Dr. Kotecha Mita

ABSTRACT

Background: Guggulu (commiphora wightii Bhandari) is a very important plant widely used for its antihyperlipidaemic, analgesic and anti- inflammatory actions by both Ayurvedic contemprory medicinal practionars. The Oleo-gumresin is a very widely used raw material of this medicinal plant and it is commonly used in Vata roga and Medo roga in Ayurveda. Objective: The current research paper provides a detailed report of comparative study the Anti -Inflammatory activity of Guggulu resin & its stem bark extract in albino rats. Methods: Test Groups of 6 rats will give a dose of the aqueous extract and Methanol Extract Commiphora wightii Bandari Stem Bark orally. After 1h, 0.1 ml, 1% carrageenan suspension in 0.9% NaCl solution was injected into the sub-plantar tissue of the right hind paw. The linear paw circumference was measured at hourly interval for 3 h. Anti-inflammatory activity will measured as the percentage reduction in oedema level when drug was present, relative to control .The increase of paw volume after 3 or 6 h is calculated as percentage compared with the volume measured immediately after injection of the irritant for each animal. Effectively treated animals show much less edema. The difference of average values between treated animals and control groups is calculated for each time interval and statistically evaluated. The difference at the various time intervals gives some hints for the duration of the inflammatory effect. A dose-response curve is run for active drugs and ED50 value can be determined. Results: All three test sample provided significant effect as anti-inflammatory agent. Conclusion: It can be concluded that all three test sample possess significant anti-inflammatory activity. Methanolic extract of stem bark and crude resin extract showed near about same effect, but aqueous extract of stem bark produced less effect than both of them.

Keywords: Guggulu resin, Guggulu stems bark, anti-inflammatory activity, Methanolic extract.