Abstract

ANTIBACTERIAL AND ANTIFUNGAL EFFECT OF CHICKPEA (CICER ARIETINUM) AQUEOUS SEED EXTRACT

Ladan Barari, Narges Mosavi, Fariba Asgharpour, Asadollah Asadi, Zahra Moulana, Maryam M. Elmi*

ABSTRACT

In the present study, 2D electrophoresis, antibacterial and antifungal effects of partially ammonium sulphat precipitation of chickpea (Cicer arietinum) seed proteins were surveyed. The antibacterial activities were evaluated by determining the zone inhibitor, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). The 2D electrophoresis showed iso-electric points of chickpea proteins from pH 5 to 7.5, which was corresponding to neutral and was slightly acidic. Also, the results showed that antibacterial activities towards Gram-positive strains (S. aureus, B. subtilis) were more active than Gram-negative strains (E.coli, P. aeruginosa and K. pneumoniae). Furthermore, B. subtilis was found to be the most sensitive strain based on MBC value. The antifungal effects on R. Solani were estimated by inhibition mycelial growth test. Inhibition mycelial growth test indicated that only 5% ammonium sulphat precipitation proteins inhibited mycelial growth with an IC50 of 60 μg ml-1.

Keywords: Chickpea, anti-proliferative, plant extract protein, cell line, anticancer.