Abstract

COMPARATIVE EVALUATION OF THE ANTIOXIDANT AND ANTIDIABETIC ACTIVITY OF DIFFERENT PARTS OF AEGLE MARMELOS AND ITS CHEMICAL PROFILING USING HPLC AND HPTLC TECHNIQUE.

Kavitha. S. Kumar , Asish G.R , Rajesh S Mony , A. Sundaresan and V. V. Venugopalan*

ABSTRACT

Aegle marmelos L. Correa commonly known as Bael belonging to the family Rutaceae has been widely used in Indian medicine due to its various medicinal properties. The roots of the plant are widely used in many ayurvedic formulations and hence trade data collected over years has indicated that the demand has exceeded the supply. Therefore it is necessary to screen the bio active phytocompounds present in all other parts of this plant so as to explore a possibility to replace the roots by substitution with other parts. Hence a comparative evaluation viz chemical and biological screening is hereby attempted to find out whether there exist any identical bio active compounds in various parts of this plant. The major focus of the present study is to investigate on the in vitro antioxidant and α-glucosidase inhibitory activity of aqueous extracts prepared from various parts of Aegle marmelos and also to perform the chemical profiling by HPLC and HPTLC techniques. A number of methods were employed for this investigation, including DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging, total polyphenol content and inhibition of α-glucosidase enzyme. Results showed that aqueous leaf extract showed antioxidant and anti-diabetic activities similar to root extract and chemical profiling showed the presence of Umbelliferone in the bark extract as well as root extract. Thus we conceded out a systematic record on the comparative antioxidant study and chemical profiling of different parts of Aegle marmelos.

Keywords: Aegle marmelos, antioxidant, ?-glucosidase, HPLC, HPTLC, Umbelliferone.