Abstract

CYTOTOXIC POTENTIALS OF ALKALOID EXTRACT OF SOLANUM KHASIANUM BERRIES AGAINST DALTON’S LYMPHOMA, MCF-7 AND HELA CELL LINES IN VITRO

Gabriel Rosangkima* and Ganesh Chandra Jagetia

ABSTRACT

In this study the cytotoxic potential of alkaloids extracted from Solanum khasianum berries was investigated against Dalton’s lymphoma, MCF-7 and HeLa cells in vitro. Cytotoxicity was determined by MTT assay and also by trypan blue dye exclusion method. The result of present study showed that one of the alkaloid extract (SKF-Alk-2) exhibited concentration-dependent cytotoxicity in all the cell types in vitro with IC50 values of 19.76 μg/ml, 32.46 μg/ml and 36.82 μg/ml against DL, MCF-7 and HeLa cells respectively. The alkaloid extract also induced a significant increase in cancer cell apoptosis at a concentration of 20 μg/ml and above. Thin layer chromatography of SKF-Alk-2 showed two distinct spots on TLC plate with the Rf value of 0.81 and 0.91. However, further fractionation of SKF-Alk-2 by open column chromatography did not resulted to produce fractions with potent cytotoxicity suggesting the synergistic effect of SKF-Alk-2.

Keywords: Solanum khasianum, alkaloids, cytotoxicity, apoptosis, synergistic effect.