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Abstract

OPTIMIZATION OF MEDIUM COMPOSITION FOR BIOMASS PRODUCTION OF NEOLENTINUS KAUFFMANII USING RESPONSE SURFACE METHODOLOGY

Johnsy. G* and V. Kaviyarasan

1Center for Advanced studies in Botany, University of Madras, Maraimalai campus, Chennai, Tamilnadu, India.

ABSTRACT

The optimization of submerged culture conditions and nutritional requirements was studied for the production of biomass from Neolentinus kauffmanii using the statistically based experimental design in a shake flask culture. Maximum mycelial biomass was observed at 25ºC. The optimal initial pH for the production of mycelial biomass was found to be pH4.0 and pH6.0, respectively. The coefficient of determination (R2) for the model was 98.5%. Probability value (P

Keywords: Response surface methodology, Neolentinus kauffmanii, biomass, probability value, regression.


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