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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2024 Issue has been successfully launched on 1 NOVEMBER 2024.

Abstract

A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ISOSORBIDE DINITRATE AND HYDRALAZINE IN BULK AND PHARMACEUTICAL DOSAGE FORM.

S. Annapurna Priyadarshika*, Dr. M. Prasada Rao, M. Srikanth

ABSTRACT

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Isosorbide dinitrate and Hydralazine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 150mm, 5μm) column using a mixture of Methanol: TEA pH 4.2 (40:60) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 272 nm. The retention time of the Isosorbide dinitrate and Hydralazine was 2.781, 4.048 ±0.02min respectively. The method produce linear responses in the concentration range of 5-25mg/ml of Isosorbide dinitrate and 9.375-46.875 mg/ml of Hydralazine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

Keywords: Isosorbide dinitrate; Hydralazine; RP-HPLC; Validation; Tablet Dosage forms.

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