Abstract

PHOSPHATE SOLUBILIZING POTENTIALITY OF BACILLUS SUBTILIS AND PSEUDOMONAS AERUGINOSA ON VIGNA UNGUICULATA GROWTH USING AGROWASTE AS A SUBSTRATE

M. Kannahi* and R. Megala

ABSTRACT

In the present study, phosphate solubilizing microorganisms were isolated from the rhizosphere soil. The phosphate solubilizing microorganisms such as Bacillus subtilis and Pseudomonas aeruginosa were identified based on cultural, morphological and biochemical characteristics. The agrowaste such as Rice Bran (RB), Wheat Bran (WB), Black Gram Husk (BGH) and Farm Yard Manure (FYM) were decomposed by the treatment of bacterial strains namely, Bacillus subtilis and Pseudomonas aeruginosa for phosphate solubilizing activity. Two set of treatments were carried out and analysed. The first set of treatment was performed with partially decomposed agrowaste, phosphate solubilizing bacteria and sowing with seeds. The second set of treatment was performed with raw agrowaste and phosphate solubilizing bacteria. After 15 days of decomposition, the seeds were sown in the second set of pots. In both set of treatment, the morphometric and phytochemical parameters were analysed at different intervals (30th, 45th and 60th days).

Keywords: Agrowaste, Decomposition, Phosphate Solubilizing Bacteria, Biochemical test, Vigna unguiculata.