Abstract

PHARMACEUTICAL ANALYSIS USING N-BROMO SUCCINAMIDE- METHYL ORANGE DYE COUPLE: A SPECTROPHOTOMETRIC STUDY

Sailaja Battu and Venkateshwarlu Gandu*

ABSTRACT

Simple, specific, accurate and precise UV–visible spectrophotometric methods have been developed for the estimation of five drugs viz.,Trimetzedine di hydrochloric acid, Esmolol hydrochloride, Tramadol, Duloxetine and Sumatriptan succinate. These methods involve the addition of a known excess of N-Bromo succinamide to the drugs in acid medium followed by estimation of residual N-bromo succinamide by reacting with a fixed amount of Methyl Orange and measuring the absorbance of the dye at 507nm. The proposed methods were found to be successful for the estimation of these drugs in bulk and their formulations. The results of analysis have been validated statistically for linearity, accuracy, and precision, Limit of detection and Limit of quantification.

Keywords: N-Bromo succinamide, Methyl orange, Spectrophotometry, Drugs.