Abstract

SCREENING OF CYPROHEPTADINE FOR ITS ANTIINFLAMMATORY POTENTIAL IN ALBINO RATS

*M.Brahadeesh, Suresha RN, Rajkishore Mahato, Thamilarasi.S, Vallish.B.N, Mohan.J

ABSTRACT

OBJECTIVE: To study the anti-inflammatory activity of cyproheptadine in acute and sub acute experimental animal model. METHODS: Adult albino rats of either sex weighing 150-200 grams were randomly divided into 3 groups of 6 each.The control,standard and test group received Gum acacia 2g%,indomethacin 10 mg/kg and cyproheptadine 2.16 mg /kg respectively .Acute inflammatory activity was assessed by carageenan induced paw oedema,Turpentine induced arthritis and sub acute activity was assessed by cotton pellet induced granuloma model. RESULTS: The anti-inflammatory activity was expressed as percentage of inhibition. In carageenan induced paw oedema, percentage of inhibition of paw oedema by indomethacin and cyproheptadine with respect to control were 51% and 21% respectively. Hence the anti-edema effect of the test group was good and comparable with standard. The percentage of inhibition of paw oedema by the test group considering the percentage of inhibition of standard as 100 % was 61.9%.Thus cyproheptadine showed good anti-inflammatory activity compared with the standard drug indomethacin in carageenan induced rat paw oedema model. The percentage of inhibition of knee arthritis with respect to control by standard and cyproheptadine were 60% and38% respectively. Hence the test group showed moderate antiarthritic effect in comparision to indomethacin .The percentage of inhibition of knee arthritis by the test group considering the percentage of inhibition of standard as 100% was 64.18%%.Thus cyproheptadine showed moderately good anti-inflammatory activity compared with the standard drug indomethacin in Turpentine induced arthritis animal model. The percentage of inhibition of granuloma by standard and cyproheptadine were50% and 32%respectively.The antigranuloma effect of test group was good as compared with standard. The percentage of inhibition of dry granulation tissue of the test group considering the percentage inhibition of standard as 100% was73.8%. Thus cyproheptadine showed significant anti-inflammatory effect compared with the standard drug indomethacin in cotton pellet induced granuloma animal model. CONCLUSION: As cyproheptadine showed considerably good antiinflammatory effect in acute models and in sub acute model, it can be used as a promising antiinflammatory agent in inflammatory conditions associated with or without allergic reactions.

Keywords: cyproheptadine, antinflammatory, indomethacin, carrageenan, turpentine, cotton pellet.