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A NEW CHROMOGENIC SPRAY REAGENT FOR DETECTION OF ORGANO PHOSPHOROUS INSECTICIDES CONTAINING NITROPHENYL GROUP BY TLC IN BIOLOGICAL MATERIALS
S.S. Prabhavale, Dr.N.L.Chutke* and Dr.M.K.Malve
ABSTRACT A new chromogenic spray reagent has been developed for the characterization of organo- phosphorous insecticide containing nitrophenyl group by thin layer chromatography. These insecticides on reduction with stannous chloride in 50 % hydrochloric acid give respective amino derivatives. These amino derivatives further react with iminodibenzyl yield purple coloured complex. Other organophosphorous, organochloro, carbamate and synthetic pyrithriod insecticides do not reacts with this reagents. Hence it is a simple and selective chromogenic reagent for organophosphorous insecticides containing nitrophenyl group,. The method has been successfully employed for the detection of Ethyl Parathion, Methyl Parathion and Fenitrothion in biological samples. Keywords: TLC, Biological tissue, Ethyl Parathion, Methyl Parathion, Fenitrothion, Iminodibenzyl. [Download Article] [Download Certifiate] |