Abstract

VALIDATED UV-SPECTROPHOTOMETRIC ABSORBANCE CORRECTION METHOD FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL, PAMABROM AND DICYCLOMINE HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM.

Hemraj Sharma*, Kammili Vishakha, K. Vinod Kumar and Nim Bahadur Dangi

ABSTRACT

Simple and rapid Absorbance correction UV-spectrophotometry method was developed for the determination of Paracetamol, Pamabrom and Dicyclomine hydrochloride, using Photo Diode Array Detector. Tablet PAMASPAS containing 325, 25, 10mg Paracetamol, Pamabrom and Dicyclomine Hydrochloride respectively was analysed for assay study. The detection was carried out at three different wavelengths 295nm, 230 nm and 217nm for Paracetamol, Pamabrom, Dicyclomine Hydrochloride, respectively by Absorbance Correction Method. The final stock solution of the Formulation mixture contained very less concentration of Dicyclomine HCL, and contains less chromophore; hence Assay was performed by Standard addition method. Calibration curve was linear (r2=0.999) in the range of 6.5-39 𝜇g/mL for Paracetamol, r2=0.999 in the range of 0.5-3 𝜇g/mL for Pamabrom and r2=0.998 in the range of 100-600 𝜇g/mL for Dicyclomine hydrochloride. The percentage recovery was found in the range of 99.05-100.38% for Paracetamol, 99.09-99.50% for Pamabrom and 98.00-99.79% for Dicyclomine Hydrochloride. Several spectroscopy methods have been developed for simultaneous estimation of these drugs with other in combination but because of unavailability of simultaneous estimation of these three drugs by UV, literatures supports for the development of this new method.The proposed method can be effectively applied for the routine analysis of Paracetamol, Pamabrom and Dicyclomine Hydrochloride in bulk and combined dosage form.

Keywords: Paracetamol, Pamabrom, Dicyclomine hydrochloride, Absorbance correction, Standard addition.