Abstract

DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY UV-VISIBLE SPECTROMETRY METHOD FOR THE DETECTION OF QUINAPYRAMINE SULFATE IN RABBIT PLASMA

Meenu Chopra, Nitu Singh Rathore, Sandeep Kumar, Balvinder Kumar, Deepti Pandita, Anju Manuja*

ABSTRACT

Quinapyramine sulfate (QS) is the most common and effective drug used for treatment of Surra. This is the first report for developing a sensitive high performance liquid chromatographic method (HPLC) UV-visible spectrometry assay for the determination of QS. A simple and robust HPLC method with UV-visible detection for QS in rabbit plasma has been developed. The QS in plasma samples was resolved by reverse-phase chromatography on a Shimadzu-HPLC system equipped with UV/VIS detector using column Inertsil ODS C18, 5 μm (150 x 4.6 mm). Analytes were separated at 1 ml/min with a linear gradient from 40% H2O/60% acetonitrile/ 0.1% formic acid over 5 min and quantified by UV absorbance at 296 nm. Recoveries at concentration of QS ranged from 2.5 to 100,00,00 ng/ml were observed between 71.45 % and 90.23%. This high performance liquid chromatographic method for the determination of QS in animal plasma can reliably quantitate concentrations of QS as low as 1.7 ng/mL.

Keywords: .