BIOSYNTHESIS OF SILVER NANOPARTICLES USING CHINESE WHITE GINSENG PLANT ROOT PANAX GINSENG
A Babu Vimalanathan, Vishakha Tyagi, A Rajesh, Preethi Devanand, *Manoj G Tyagi
Department of Pharmacology + & Department of Biotechnology* Christian Medical College, Vellore 632002+ & Vellore Institute of Technology*, Vellore, Tamilnadu, India.
ABSTRACT
Nanotechnology is an emerging field of nano science, and utilizes nano
based systems for various biomedical applications. This rapidly
developing field of nanoscience has raised the possibility of using
nanoparticles in the diagnosis, treatment and therapeutics. The use of
plant products for the synthesis of nanoparticles adds a new dimension
for modern nanobio-technology. The cost effectual and environmently
safe technique for the green synthesis of silver nanoparticles using the
extract of Chinese white ginseng-Panax ginseng plant root extract as
reducing and capping agent from one milli molar silver nitrate solution
is described in this article. Objectives: The objective of this study is to
present an evidence-based eco-friendly perspective of silver
nanoparticles properties in synthesis and applications. The Biomedical
application of silver nanoparticles can be rendered more effective by using biologically
synthesized nanoparticles that are found to be exceptionally stable, and also minimize
toxicity and cost. This study illustrates the synthesis of silver nanoparticles using the Chinese
white Ginseng plant root –Panax ginseng after 24 h of incubation period at room
temperature. Methods: These silver nanoparticles were determined and characterized by
means of UV-Vis Spectroscopy, Scanning Electron Microscopy (SEM) and X-ray diffraction
(XRD).The nanoparticles exhibited maximum absorbance at 500nm in UV-Vis Spectroscopy.
The XRD Spectrum of Silver nanoparticles exhibited two (2) theta values corresponding to
the silver nanocrystals. SEM Micrographs revealed the formation of well-dispersed silver
nanoparticles of 100 approximately nm size. Results: The Debye-scherrer equation was used
to calculate particles sizes, and silver nanoparticles of about 100 nm were observed. The process of Ag+ reduction is plant bio-reduction which made it an easy, reliable and ecofriendly
(Green synthesis) method for the synthesis of silver nanoparticles for therapeutic
purpose.
Keywords: Silver nanocrystals, XRD, SEM, UV-vis, green synthesis.
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