Abstract

“ISOLATION OF DYE DEGRADING PSEUDOMONAS SPECIES FROM THE SOIL NEAR DYEING INDUSTRY ERODE AND ITS POTENTIAL APPLICATION IN DYE EFFLUENT TREATMENT”

Tariq Ahmad Lone*, C.Revathi and Reyaz Ahmad Lone

ABSTRACT

The dye related environmental pollution is increasing at an alarming rate over the worldwide especially in India. This made us to study on the biological control of dye pollutants by isolating the dye degrading microbes from contaminated soil. A total of seven dye degrading bacteria were isolated. Among these the most potential isolate S2 was further characterized. It was found to be a gram negative, rod shaped, flagellated, motile bacteria. The bacterial isolate showed positive reaction for the methyl red, voges proskaure and citrate while negative reaction for the indole test. Degrading strain S2 fermented glucose only while did not showed fermentations in the lactose, sucrose, fructose, xylose, arabinose, sorbitol and rhaminose. The degrading strain S2 showed the zone of hydrolysis gelatin agar medium, casein agar medium and did not showed the hydrolysis in the starch agar medium. Degrading strain S2 showed the positive reaction for the oxidase test, catalase test, urease test, and nitrate reduction test. Thus the identification test profile for the dye degrading strain S2 made it confirmed that isolated strains were belonging genus Pseudomonas species. The physical and chemical parameters showed the highest decolorizing activity were found at pH 6 (89.06%) and lowest decolorizing activity at pH 9 (82.34%). The effect of temperature for dye degrading strain Pseudomonas species S2 showed highest decolorizing activity at 370C (90.08%) and lowest decolorizing activity at 450C (71.65%). The Pseudomonas species S2 showed good ability to decolorize the dyes Red m 5B and T-Blue upto 300ppm. The dye degrading Pseudomonas species showed good ability to decolorize the dyes on metabolic shaker when compared to under static condition. Strain improvement for the selected isolate of dye degrading Pseudomonas species strain S2 showed that fewer colonies appeared on Petri plates with an increasing exposure to Ultra Violet light and similar result was observed with increasing concentration of Ethidium Bromide, the growth rate decreased as only few colonies appeared on the Petriplates.

Keywords: Pseudomonas species, Effluent treatment, Potential Dye Degrading Strain, oxidase positive.