Abstract

ANALGESIC STUDY OF METHANOLIC EXTRACTS OF LORANTHUS EUROPAEUS LEAF AND STEM

Fatema Nasrin, Md. Lukman Hakim*, Reaz Muhammad Pinon, Mou Chakraborty, Nane Gopal Sarker, Khurshad Alam

ABSTRACT

In this study the analgesic potency of methanolic extracts of Loranthus europaeus leaf (200 & 400 mg/kg body weight) and stem (200 & 400 mg/kg body weight) was evaluated by applying acetic acid induced writhing and tail immersion method in mice. To determine the peripheral analgesic activity acetic acid induced writhing method was adopted, Diclofenac sodium (10 mg/kg body weight) served as standard. 1% acetic acid (0.1 ml/10 gm) was injected intraperitoneally after 30 minutes of the administration of the extracts, 5 minutes later writhing number of different groups were counted for next 15 minutes. All extracts yielded potent analgesic activities by achieving reduced writhing numbers compared with Control. Among the extracts the highest writhing inhibition (59.66%) was recorded for 400 mg/kg body weight of leaf extract. In tail immersion test to perform central analgesic efficacy, tail withdrawing times of different groups were observed after 0, 30, 60, 90 and 120 minutes of the administration of extracts. The popular analgesic Indomethacine (10 mg/kg body weight) served as standard. The extracts exerted analgesic efficiency by ensuring elongated tail withdrawing time compared with Control. One Way Anova followed by Dunnett’s multiple comparison test was performed to evaluate the statistical significance of the values. In both tests, experimental extracts achieved statistical significant P value (P

Keywords: Loranthus europaeus, analgesic, writhing, acetic acid induced writhing method, tail immersion test.