Abstract

16S RDNA IDENTIFICATION AND GENBANK DEPOSITION OF ACINETOBACTER SP. STRAIN PR-6 CAPABLE OF GROWING IN EXPLOSIVE LADEN SOIL WITH BLAST TREE VIEW

P. Ravikumar PhD*

ABSTRACT

Ribosomal deoxyribonucleic acid sequencing, polymerase chain reaction and deoxyribonucleic acid sequencing has played a pivotal role in the accurate identification of bacterial/fungal isolates and the discovery of novel bacteria with their 16S rDNA and fungi with their 18S rDNA sequencing in explosive laden soil. Ten different Bacterial isolates and three different Actinomycetes belongs to the genera Acinetobacter, Bacillus, Enterobacter, Enterococcus, Staphylococcus, klebsiella, Aspergillus, Coriolopsis were isolated and identified with their 16S and 18S rDNA sequences and deposited in the The GenBank Maryland USA and MycoBank Utrecht Netherlands. All the isolates were named after the discoverer P Ravikumar, will be preserved in MTCC, India. Sanger dideoxy sequencing technology was employed and the number of base pairs, the base count of A, T, G and C was also studied. To fully utilise 16S/18S rDNA sequencing of bacteria and fungi in explosive laden soils and their bioremediation, the presence of cat, ben, xplA, xplB and other biodegrading gene/s, catabolic genes and their gene cassettes are being investigated. Acinetobacter sp. Strain PR-6 16S ribosomal RNA gene, with the base count 218 a 279 c 182 g 215 t partial sequence with Accession KP261384, Version KP261384.1 GI:758375282 (bases 1- 894), a novel strain present in the explosive laden soil of cracker industry was deposited in the The GenBank Maryland USA is discussed here.

Keywords: Explosive laden soil, Acinetobacter sp.strain PR-2, 16S rDNA sequence, Specific PCR, Novel strain, Discovery, The GenBank deposition.