Abstract

ANTI-OXIDATIVE AND ANTI-DIABETIC EFFECT OF POD EXTRACT OF PITHECELLOBIUM DULCE (ROXB.) BENTH, ON LIVER, KIDNEY AND PANCREAS IN ALLOXAN INDUCED DIABETIC MALE SWISS ALBINO MICE

Swaati Sharma*, Nitika Verma and Veena Garg

ABSTRACT

Pithecellobium dulce has previously been used for the treatment of various diseases. In this study the pod extract of P. dulce is being used to investigate the anti- oxidative and anti-diabetic activity in alloxan induced diabetic male Swiss albino mice and the tissues were also studied for histo-pathological changes induced after induction of diabetes and subsequent extract treatment. 24 male Swiss albino mice each of 25-32g of body weight were divided in 4 groups (each group was maintained with 6 mice), I Normal Control (NC), II Diabetic Control (DC), III Glibenclamide Treated (GT) and IV P. dulce pod extract treated group (PDPE). Diabetes was induced to DC, GT and PDPE by a single intraperitoneal injection (IP) of alloxan monohydrate of 150mg/kg B.w. Group IV was orally administered by a single dose of 300mg/kg B.w./d of PDPE extract, whereas diabetic group III was treated with glibenclamide (10mg/kg of B.w.) for 45 days. Fasting Blood Glucose levels of all the four groups were measured using blood glucose test strips of Dr. Morpen‟s glucometer at a regular interval of 0, 15, 30 and 45th days and various tissue homogenates viz liver, kidney, pancreas was used for the estimation of enzymatic antioxidants such as Superoxide dismutase (SOD), Catalase (CAT) and Glutathione peroxidase (GPx). Hepatic, Renal and Pancreatic tissues from the four groups were studied for histological changes, induced after Alloxan injection and subsequent extract treatment. Data obtained from the study shows significant (P

Keywords: Diabetes mellitus, Antioxidants, Antidiabetic, Histopathology, Alloxan and Glibenclamide.