Abstract

MULTIDRUG RESISTANCE PATTERN OF EXTENDED SPECTRUM ?-LACTAMASE PRODUCING KLEBSIELLA SPECIES ISOLATED FROM URINE SAMPLES

V. A. Kamble*, M. G. Thosar and C. S. Ukesh

ABSTRACT

Urinary tract infection (UTI) is most common in outpatient settings and hospitalized patients. Klebsiella is one of the major pathogen causing UTIs. These bacteria inactivate the penicillins and cephalosporins by producing extended spectrum β-lactamases (ESBL). The present research aimed to observe the prevalence of ESBL producing Klebsiella species isolated from urine specimens and to study their multidrug resistance pattern in Amravati and Akola cities. A total of 385 urine samples were collected form Amravati and Akola cities. All samples were taken from prescribed patients and processed according to standard operating laboratory procedures. Isolates were identified by standard conventional biochemical methods and checked for ESBL production according to NCCLS criteria and antimicrobial susceptibility was performed by Kirby-Bauer disc diffusion method. Total 165 Klebsiella were isolated from 385 urine specimens, among them 142 isolates were Klebsiella pneumoniae, 22 were K. oxytoca and only one isolate had K. ozanae. Total 24.24% of Klebsiella were found to be ESBL positive. ESBL positive Klebsiella showed 100% resistance to ampicillin followed by 90.32% resistance to penicillin –G, amoxicillin, augumentin and Kanamycin, while lowest resistance 3.23% to meropenem and chloramphenicol. The result of this study showed a significant prevalence of multidrug resistant ESBL producing Klebsiella and will help in the empiric therapy of infection caused by Klebsiella species. Laboratory evidence of infection and antibiotic susceptibility testing should be carried out to help in the choice of systemic drug which may help in minimization of emergence of drug resistant bacteria.

Keywords: UTI, Klebsiella, multidrug resistance, ESBL.