Abstract

ACHYRANTHES ASPERA HAVE THE POTENTIAL ANTIOXIDANT PROPERTY IN SCAVENGING FREE RADICALS PRODUCED AS A RESULT OF OXIDATIVE STRESS INDUCED BY ARSENIC.

Shayan Shakeel* Ajay Kumar Sharma* Md Sohail* and Veena Sharma**

ABSTRACT

The current investigation was undertaken to evaluate antioxidant potential role of Achyranthus aspera in arsenic induced oxidative stress in swiss albino mice. In this study, impact of two different plant part (root and leaves) of Achyranthes aspera on adult female mice which were treated with various plant extracts following exposure to arsenic was investigated. 48 Swiss albino mice weighing 20-30g were obtained from Haryana Agricultural University, Hissar, India and were divided into 6 groups each containing 8 mice and received various doses of leaf and root extracts following sodium arsenate treatment. Liver was obtained from the mice and tissue homogenates were used for the estimation of SOD, CAT, GSH, GPx, GST and LPO. Arsenic exposure led to significant rise TBARS (0.313± .002 nmols TBARS/h/g), fall in SOD (0.190± .003 U/mg protein/h), decrease in CAT activity (0.329 ± .001 nmol H2O2 consumed/min/mg protein), rise in GSH (0.072± .005 nmol GSH / g tissue) and significant decrease in GST activity (2.81 ± .14 nmols CDNB conjugates formed/min/mg protein) as compared to control group. From the above findings, it can be concluded that, Achyranthes aspera have the anti-oxidant potential in scavenging free radical produced as a result of oxidative stress induced by arsenic.

Keywords: Achyranthes aspera, anti-oxidant potential.