Abstract

ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF FRACTIONATED PERSEA GRATISSIM SEED EXTRACTS

Vedamurthy AB, Padmaa M Paarakh* and Sudisha Jogaiah

ABSTRACT

Fractionated methanol extracts of dried seeds of Persea gratissim were evaluated for the antibacterial and antioxidant activity. The extracts were tested for antibacterial activity by spread plate method against Bacillus subtilis and Streptococcus aureus. The antioxidant activity of these fractions were determined by DPPH [1, 1-Diphenyl-2-Picryl Hydrazyl] assay. Fraction III and IV showed a maximum inhibition zone against Bacillus subtilis and Staphylococcus aureus at 50 μl concentration. The antioxidant activity of the seed extracts were observed to scavenge 1,1-diphenyl,2-picrylhydrazyl(DPPH) in the order terpenoids > lipids > alkaloids > polysaccharide. Among the four fractions analyzed, maximum antioxidant activity was observed in fraction III and recorded 81.6% compared to standard antioxidants ascorbic acid [82.3%]. Moderate antioxidant was observed in fraction I,II and IV.

Keywords: Avocados, Antibacterial, Antioxidant, DPPH, Bacillus subtilis, Persea gratissim, Staphylococcus aureus.