Abstract

ANTIPROLIFERATIVE EFFECT OF BULGARIAN SPRING WATER PROBIOTICS (LAKTERA NATURE PROBIOTIC®) AGAINST HUMAN COLON CARCINOMA CELL LINE

Kaloyan Georgiev*, Marieta Georgieva, Ivan Iliev, Maria Peneva, Georgi Alexandrov

ABSTRACT

Colorectal carcinoma is a malignant disease, from which more than 1 million people worldwide suffer. Over the past two decades, its social significance attracts more attention. Colorectal cancer is the second leading cause of death from malignancy and the third most frequently diagnosed malignancy. It has been estimated that nutrition is the main reason for the high incidence of cancer. Probiotics are live microorganisms with proven health benefits in a variety of gastrointestinal diseases, and disorders outside the gastrointestinal tract. The aim of the present study is to explore cytotoxic and antiproliferative effects of the new Laktera Nature Probiotic® on noncancerous cell lines (BALB/3T3 and BJ) and colon adenocarcinoma cell line – HT-29. Laktera Nature Probiotic® has weak direct cytotoxicity against BALB/3T3 and BJ cells, but inhibits their proliferation in a dose-dependent manner. On carcinoma cell line – HT-29, in low concentration range (2-125 μg/ml) Laktera Nature Probiotic® stimulates proliferation; at high concentrations (250-1000 μg/ml) we detected concentration-dependent inhibition of proliferation. Laktera Nature Probiotic® has shownpromising primary screening results, which should be clarified as mechanisms in further works.

Keywords: probiotics, cytotoxicity, colon carcinoma, antiproliferative effect.