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METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF SITAGLIPTIN AND SIMVASTATIN IN PHARMACEUTICAL FORMULATION BY HPTLC
Malathi S* and Sivakumar T
ABSTRACT
This article gives a simple, rapid and accurate High performance thin layer chromatographic method for determination of Sitagliptin and Simvastatin in pharmaceutical formulation. Merck TLC aluminum sheets of silicagel G60 F254 with the thickness of 200 μm was used to carry out the separation. Ethyl acetate: toluene: methanol (6:2:2 v/v/v), was used as mobile phase. Analysis of the compounds was carried out by densitometry in the absorbance mode at 254 nm. The selected mobile phase gave the well defined peak at the Rf value of 0.6 +0.02 and 0.3+0.02 for sitagliptin and simvastatin respectively. Method was then validated for accuracy, linearity, precision, specificity, limit of Keywords: Sitagliptin, Simvastatin, HPTLC, Validation. [Download Article] [Download Certifiate] |