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Abstract

NEW SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF THIOCOLCHICOSIDE IN BULK AND PHARMACEUTICAL FORMULATIONS

*Chinna Raja.Pydi, Nirmala Jyothi.P, Pallavi.P, Rafath Sulthana.Md, Kiran Kumar.G

ABSTRACT

Three simple spetrophotometric methods (A, B and C) have been described for the assay of Thiocolchicoside in bulk and in pharmaceutical formulations. In method A Thiocolchicoside is condensed with P-dimethylaminobenzaldehyde (PDAB) to form a yellow coloured Schiff’s base having an absorption maximum (λmax) of 400nm. Method B is based on the oxidation / reduction reaction between the Thiocolchicoside and hydrochloride reagent to form a yellow coloured chromogen with λmax at 400 nm. Method C involves oxidation of Thiocolchicoside with ferric chloride followed by coupling with N-methylbenzothiazolone hydrazone (MBTH) to form yellowish green coloured complex with λmax at 410nm.These methods have been statistically evaluated and are found to be precise and accurate.

Keywords: Thiocolchicoside, MBTH, Validation, UV-Visible.


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